› 4lmf

X-ray diffraction
2.92Å resolution

Function and Biology Details

Reaction catalysed: 
Cleavage of Arg-|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement C1s subcomponent heavy chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 31.12 KDa
Source organism: Homo sapiens
Expression system: Cricetinae
UniProt:
  • Canonical: P09871 (Residues: 17-292; Coverage: 41%)
Gene name: C1S
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 67.876Å b: 71.668Å c: 157.723Å
α: 90° β: 95.93° γ: 90°
R-values:
R R work R free
0.211 0.208 0.252
Expression system: Cricetinae

Quick links

Citations

13 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
12 more

4 mentions without citation

Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.
Mortensen et al. (2017)
3 more