› 4lny

X-ray diffraction
1.93Å resolution

Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR422

Released:
DOI: 10.2210/pdb4lny/pdb
PDB entry 4lny coloured by chain, front view.
PDB entry 4lny coloured by chain, side view.
PDB entry 4lny coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Vorobiev S, Su M, Bjelic S, Kipnis Y, Wang L, Sahdev S, Xiao R, Maglaqui M, Kogan S, Baker D, Everett JK, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 30.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06121 (Residues: 1-234, 235-247; Coverage: 100%)
Gene names: SSO0895, trpC
Sequence domains: Indole-3-glycerol phosphate synthase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CD 6 x CD
Ligand CL 5 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 41.125Å b: 63.161Å c: 86.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.222
Expression system: Escherichia coli BL21(DE3)

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