› 4ls6

X-ray diffraction
1.56Å resolution

Crystal structure of beta-ketoacyl-ACP synthase II (FabF) I108F mutant from Bacillus subtilis

Released:
DOI: 10.2210/pdb4ls6/pdb
PDB entry 4ls6 coloured by chain, front view.
PDB entry 4ls6 coloured by chain, side view.
PDB entry 4ls6 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural insights into bacterial resistance to cerulenin.
Trajtenberg F, Altabe S, Larrieux N, Ficarra F, de Mendoza D, Buschiazzo A, Schujman GE
FEBS J 281 2324-38 (2014)
PMID: 24641521

Function and Biology Details

Reaction catalysed: 
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 45.64 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34340 (Residues: 1-413; Coverage: 100%)
Gene names: BSU11340, fabF, yjaY
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand K 3 x K
Ligand GOL 5 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 72.069Å b: 87.974Å c: 145.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.149 0.172
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The DSF Family of Quorum Sensing Signals: Diversity, Biosynthesis, and Turnover.
Zhou et al. (2017)
2 more

1 mention without citation

Structural insights into bacterial resistance to cerulenin.
Trajtenberg et al. (2014)