› 4lsi

X-ray diffraction
2.09Å resolution

Ion selectivity of OmpF porin soaked in 0.3M KBr

Released:
DOI: 10.2210/pdb4lsi/pdb
PDB entry 4lsi coloured by chain, front view.
PDB entry 4lsi coloured by chain, side view.
PDB entry 4lsi coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A structural study of ion permeation in OmpF porin from anomalous X-ray diffraction and molecular dynamics simulations.
Dhakshnamoorthy B, Ziervogel BK, Blachowicz L, Roux B
J Am Chem Soc 135 16561-8 (2013)
PMID: 24106986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 37.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

5 bound ligands:
Ligand PEG 9 x PEG
Ligand C8E 4 x C8E
Ligand GOL 4 x GOL
Ligand MG 8 x MG
Ligand BR 9 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 202.094Å b: 116.559Å c: 53.592Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.193 0.19 0.245
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
2 more

1 mention without citation

A structural study of ion permeation in OmpF porin from anomalous X-ray diffraction and molecular dynamics simulations.
Dhakshnamoorthy et al. (2013)