› 4lwy

X-ray diffraction
2.9Å resolution

L(M196)H,H(M202)L Double Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV

Released:
DOI: 10.2210/pdb4lwy/pdb
PDB entry 4lwy coloured by chain, front view.
PDB entry 4lwy coloured by chain, side view.
PDB entry 4lwy coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Molecular Dynamic Studies of Reaction Centers Mutants from Rhodobacter sphaeroides and his mutant form L(M196)H+H(M202)L
Kljashtorny VG, Fufina TY, Vasilieva LG, Gabdulkhakov AG
CRYSTALLOGR REP. 59 536-541 (2014)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 282 amino acids
Theoretical weight: 31.49 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 1-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 303 amino acids
Theoretical weight: 34.02 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 1-303; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

13 bound ligands:
Ligand LDA 6 x LDA
Ligand UNL 11 x UNL
Ligand PO4 3 x PO4
Ligand DIO 4 x DIO
Ligand EDO 7 x EDO
Ligand TRS 2 x TRS
Ligand K 1 x K
Ligand BCL 3 x BCL
Ligand BPH 3 x BPH
Ligand U10 2 x U10
Ligand CDL 1 x CDL
Ligand FE 1 x FE
Ligand SPN 1 x SPN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 140.04Å b: 140.04Å c: 184.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.262
Expression system: Cereibacter sphaeroides

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