› 4lxs

X-ray diffraction
3.3Å resolution

Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity (glycosylated form)

Released:
DOI: 10.2210/pdb4lxs/pdb
PDB entry 4lxs coloured by chain, front view.
PDB entry 4lxs coloured by chain, side view.
PDB entry 4lxs coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity.
Parthier C, Stelter M, Ursel C, Fandrich U, Lilie H, Breithaupt C, Stubbs MT
Proc Natl Acad Sci U S A 111 6281-6 (2014)
PMID: 24733933

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140445 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein toll Chain: A
Molecule details ›
Chain: A
Length: 783 amino acids
Theoretical weight: 89.63 KDa
Source organism: Drosophila melanogaster
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P08953 (Residues: 28-802; Coverage: 72%)
Gene names: CG5490, Tl
Sequence domains:
Structure domains: Ribonuclease Inhibitor
Protein spaetzle C-106 Chains: J, K
Molecule details ›
Chains: J, K
Length: 114 amino acids
Theoretical weight: 13.01 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P48607 (Residues: 221-326; Coverage: 35%)
Gene names: CG6134, spz
Sequence domains: Spaetzle
Structure domains: Cystine-knot cytokines

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 171.278Å b: 76.824Å c: 123.822Å
α: 90° β: 126.29° γ: 90°
R-values:
R R work R free
0.209 0.207 0.264
Expression systems:
  • Drosophila melanogaster
  • Escherichia coli

Quick links

Citations

3 review citations

The Function of Fish Cytokines.
Zou et al. (2016)
2 more

1 mention without citation

Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity.
Parthier et al. (2014)