4lyy

X-ray diffraction
1.86Å resolution

Crystal structure of hypoxanthine phosphoribosyltransferase from Shewanella pealeana ATCC 700345, NYSGRC Target 029677.

Released:
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-108645 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 23.04 KDa
Source organism: Shewanella pealeana ATCC 700345
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8H0C8 (Residues: 1-177; Coverage: 100%)
Gene name: Spea_0688
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 51.514Å b: 101.173Å c: 73.844Å
α: 90° β: 107.38° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli BL21(DE3)