› 4map

X-ray diffraction
1.9Å resolution

Crystal structure of Ara h 8 purified with heating

Released:
DOI: 10.2210/pdb4map/pdb
PDB entry 4map coloured by chain, front view.
PDB entry 4map coloured by chain, side view.
PDB entry 4map coloured by chain, top view.
Source organism: Arachis hypogaea
Primary publication:
Structure and function of the peanut panallergen Ara h 8.
Hurlburt BK, Offermann LR, McBride JK, Majorek KA, Maleki SJ, Chruszcz M
J Biol Chem 288 36890-901 (2013)
PMID: 24253038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bet v I/Major latex protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 16.97 KDa
Source organism: Arachis hypogaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VT83 (Residues: 1-157; Coverage: 100%)
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 40.922Å b: 87.113Å c: 42.863Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.2 0.197 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Food allergy: immune mechanisms, diagnosis and immunotherapy.
Yu et al. (2016)
10 more

2 mentions without citation

Structure and function of the peanut panallergen Ara h 8.
Hurlburt et al. (2013)
1 more