› 4mn8

X-ray diffraction
3.06Å resolution

Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains

Released:
DOI: 10.2210/pdb4mn8/pdb
PDB entry 4mn8 coloured by chain, front view.
PDB entry 4mn8 coloured by chain, side view.
PDB entry 4mn8 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex.
Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C, Zhou JM, Chai J
Science 342 624-8 (2013)
PMID: 24114786

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-164824 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
LRR receptor-like serine/threonine-protein kinase FLS2 Chain: A
Molecule details ›
Chain: A
Length: 782 amino acids
Theoretical weight: 85.52 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9FL28 (Residues: 25-800; Coverage: 68%)
Gene names: At5g46330, FLS2, MPL12.13, MPL12.8
Sequence domains:
BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 226 amino acids
Theoretical weight: 24.63 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q94F62 (Residues: 1-220; Coverage: 33%)
Gene names: At4g33430, BAK1, ELG, F17M5.190, SERK3
Sequence domains:
Structure domains: Ribonuclease Inhibitor
flg22 Chain: C
Molecule details ›
Chain: C
Length: 22 amino acids
Theoretical weight: 2.28 KDa

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 9 x NAG
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 108.865Å b: 113.683Å c: 164.363Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.274
Expression system: Spodoptera frugiperda

Quick links

Citations

91 review citations

Growth-defense tradeoffs in plants: a balancing act to optimize fitness.
Huot et al. (2014)
90 more

30 mentions without citation

A molecular roadmap to the plant immune system.
Bentham et al. (2020)
29 more