› 4muv

X-ray diffraction
1.25Å resolution

M. loti cyclic-nucleotide binding domain mutant displaying inverted ligand selectivity, cyclic-GMP bound

Released:
DOI: 10.2210/pdb4muv/pdb
PDB entry 4muv coloured by chain, front view.
PDB entry 4muv coloured by chain, side view.
PDB entry 4muv coloured by chain, top view.
Source organism: Mesorhizobium japonicum MAFF 303099
Primary publication:
Determinants of ligand selectivity in a cyclic nucleotide-regulated potassium channel.
Pessoa J, Fonseca F, Furini S, Morais-Cabral JH
OpenAccess logo J Gen Physiol 144 41-54 (2014)
PMID: 24981229

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-gated potassium channel mll3241 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q98GN8 (Residues: 216-355; Coverage: 39%)
Gene name: mll3241
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand PCG 2 x PCG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 60.051Å b: 61.572Å c: 67.844Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.136 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Current Structural Knowledge on the CNNM Family of Magnesium Transport Mediators.
Giménez-Mascarell et al. (2019)
4 other articles

4 mentions without citation

Pose Classification Using Three-Dimensional Atomic Structure-Based Neural Networks Applied to Ion Channel-Ligand Docking.
Shim et al. (2022)
3 more