› 4n82

X-ray diffraction
1.88Å resolution

X-ray crystal structure of Streptococcus sanguinis NrdIox

Released:
DOI: 10.2210/pdb4n82/pdb
PDB entry 4n82 coloured by chain, front view.
PDB entry 4n82 coloured by chain, side view.
PDB entry 4n82 coloured by chain, top view.
Source organism: Streptococcus sanguinis SK36
Primary publication:
Streptococcus sanguinis class Ib ribonucleotide reductase: high activity with both iron and manganese cofactors and structural insights.
Makhlynets O, Boal AK, Rhodes DV, Kitten T, Rosenzweig AC, Stubbe J
J Biol Chem 289 6259-72 (2014)
PMID: 24381172

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative NrdI-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.07 KDa
Source organism: Streptococcus sanguinis SK36
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3CR20 (Residues: 5-162; Coverage: 98%)
Gene names: SSA_2263, nrdI
Sequence domains: NrdI Flavodoxin like
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand FMN 2 x FMN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 104.726Å b: 56.612Å c: 73.387Å
α: 90° β: 106.13° γ: 90°
R-values:
R R work R free
0.208 0.207 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Manganese homeostasis and utilization in pathogenic bacteria.
Juttukonda et al. (2015)
10 more

2 mentions without citation

Streptococcus sanguinis class Ib ribonucleotide reductase: high activity with both iron and manganese cofactors and structural insights.
Makhlynets et al. (2014)
1 more