› 4n8i

X-ray diffraction
2.01Å resolution

M31G mutant, RipA structure

Released:
DOI: 10.2210/pdb4n8i/pdb
PDB entry 4n8i coloured by chain, front view.
PDB entry 4n8i coloured by chain, side view.
PDB entry 4n8i coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Structural snapshots along the reaction pathway of Yersinia pestis RipA, a putative butyryl-CoA transferase.
Torres R, Lan B, Latif Y, Chim N, Goulding CW
Acta Crystallogr D Biol Crystallogr 70 1074-85 (2014)
PMID: 24699651

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxybutyrate coenzyme A transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 52.21 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZC36 (Residues: 1-440; Coverage: 100%)
Gene name: YPO1926
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand COA 2 x COA
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 117.411Å b: 107.171Å c: 87.8Å
α: 90° β: 120.36° γ: 90°
R-values:
R R work R free
0.137 0.135 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

The activating oxydianion binding domain for enzyme-catalyzed proton transfer, hydride transfer, and decarboxylation: specificity and enzyme architecture.
Reyes et al. (2015)
1 more

2 mentions without citation

Crystal structure of a Pseudomonas malonate decarboxylase holoenzyme hetero-tetramer.
Maderbocus et al. (2017)
1 more