4nbz

X-ray diffraction
1.75Å resolution

Crystal Structure of TcdA-A1 Bound to A26.8 VHH

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-210677 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Toxin A Chains: A, C
Molecule details ›
Chains: A, C
Length: 144 amino acids
Theoretical weight: 16.29 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16154 (Residues: 2573-2709; Coverage: 5%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Cholin Binding
A26.8 VHH Chains: B, D
Molecule details ›
Chains: B, D
Length: 152 amino acids
Theoretical weight: 17.01 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P32
Unit cell:
a: 60.002Å b: 60.002Å c: 133.935Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli