› 4nhf

X-ray diffraction
2Å resolution

Crystal structure of the soluble domain of TrwG Type IV secretion machinery from Bartonella grahamii

Released:
DOI: 10.2210/pdb4nhf/pdb
PDB entry 4nhf coloured by chain, front view.
PDB entry 4nhf coloured by chain, side view.
PDB entry 4nhf coloured by chain, top view.
Source organism: Bartonella grahamii as4aup
Primary publication:
Structural Insight into How Bacteria Prevent Interference between Multiple Divergent Type IV Secretion Systems.
Gillespie JJ, Phan IQ, Scheib H, Subramanian S, Edwards TE, Lehman SS, Piitulainen H, Rahman MS, Rennoll-Bankert KE, Staker BL, Taira S, Stacy R, Myler PJ, Azad AF, Pulliainen AT
OpenAccess logo mBio 6 e01867-15 (2015)
PMID: 26646013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV secretion system protein virB8 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 180 amino acids
Theoretical weight: 20.38 KDa
Source organism: Bartonella grahamii as4aup
Expression system: Escherichia coli
UniProt:
  • Canonical: C6AAT5 (Residues: 63-233; Coverage: 73%)
Gene names: Bgr_19310, trwG
Sequence domains: VirB8 protein
Structure domains: VirB8 protein

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 62.95Å b: 87.65Å c: 191.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.24
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacteria-Killing Type IV Secretion Systems.
Sgro et al. (2019)
3 more

4 mentions without citation

Structural Insight into How Bacteria Prevent Interference between Multiple Divergent Type IV Secretion Systems.
Gillespie et al. (2015)
3 more