› 4npf

X-ray diffraction
1.49Å resolution

High-resolution structure of two tandem B domains of staphylococcal protein A connected by the conserved linker

Released:
DOI: 10.2210/pdb4npf/pdb
PDB entry 4npf coloured by chain, front view.
PDB entry 4npf coloured by chain, side view.
PDB entry 4npf coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity.
Deis LN, Pemble CW, Qi Y, Hagarman A, Richardson DC, Richardson JS, Oas TG
Structure 22 1467-77 (2014)
PMID: 25295398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 116 amino acids
Theoretical weight: 13.27 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38507 (Residues: 154-269; Coverage: 25%)
Gene name: spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 44.412Å b: 44.412Å c: 214.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.145 0.142 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Functional roles of transiently and intrinsically disordered regions within proteins.
Uversky VN. (2015)
2 more

4 mentions without citation

Multiscale conformational heterogeneity in staphylococcal protein a: possible determinant of functional plasticity.
Deis et al. (2014)
3 more