› 4ntg

X-ray diffraction
2.55Å resolution

Crystal structure of D60A mutant of Arabidopsis ACD11 (accelerated-cell-death 11) complexed with C12 ceramide-1-phosphate (d18:1/12:0) at 2.55 Angstrom resolution

Released:
DOI: 10.2210/pdb4ntg/pdb
PDB entry 4ntg coloured by chain, front view.
PDB entry 4ntg coloured by chain, side view.
PDB entry 4ntg coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels.
Simanshu DK, Zhai X, Munch D, Hofius D, Markham JE, Bielawski J, Bielawska A, Malinina L, Molotkovsky JG, Mundy JW, Patel DJ, Brown RE
Cell Rep 6 388-99 (2014)
PMID: 24412362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Accelerated cell death 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.75 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O64587 (Residues: 1-206; Coverage: 100%)
Gene names: ACD11, At2g34690, T29F13.10
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

1 bound ligand:
Ligand 1PZ 2 x 1PZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R32
Unit cell:
a: 101.507Å b: 101.507Å c: 280.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.182 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Lipid signalling in plant responses to abiotic stress.
Hou et al. (2016)
15 more

2 mentions without citation

Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels.
Simanshu et al. (2014)
1 more