› 4nxe

X-ray diffraction
2.1Å resolution

Crystal structure of iLOV-I486(2LT) at pH 6.5

Released:
DOI: 10.2210/pdb4nxe/pdb
PDB entry 4nxe coloured by chain, front view.
PDB entry 4nxe coloured by chain, side view.
PDB entry 4nxe coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Significant expansion of fluorescent protein sensing ability through the genetic incorporation of superior photo-induced electron-transfer quenchers.
Liu X, Jiang L, Li J, Wang L, Yu Y, Zhou Q, Lv X, Gong W, Lu Y, Wang J
J Am Chem Soc 136 13094-7 (2014)
PMID: 25197956

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 13.97 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P93025 (Residues: 388-496; Coverage: 12%)
Gene names: At5g58140, CAV1, K21L19.6, KIN7, NPL1, PHOT2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
1 modified residue:
Ligand 2LT 2 x 2LT

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 37.474Å b: 65.43Å c: 49.565Å
α: 90° β: 102.67° γ: 90°
R-values:
R R work R free
0.209 0.205 0.253
Expression system: Escherichia coli

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Citations

6 review citations

Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.
Jin et al. (2019)
5 more