› 4nzv

X-ray diffraction
1.9Å resolution

DNA Double-Strand Break Repair Pathway Choice Is Directed by Distinct MRE11 Nuclease Activities

Released:
DOI: 10.2210/pdb4nzv/pdb
PDB entry 4nzv coloured by chain, front view.
PDB entry 4nzv coloured by chain, side view.
PDB entry 4nzv coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities.
Shibata A, Moiani D, Arvai AS, Perry J, Harding SM, Genois MM, Maity R, van Rossum-Fikkert S, Kertokalio A, Romoli F, Ismail A, Ismalaj E, Petricci E, Neale MJ, Bristow RG, Masson JY, Wyman C, Jeggo PA, Tainer JA
Mol Cell 53 7-18 (2014)
PMID: 24316220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA double-strand break repair protein Mre11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 38.58 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1X0 (Residues: 2-324; Coverage: 84%)
Gene names: TM_1635, mre11
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 47.116Å b: 109.949Å c: 76.917Å
α: 90° β: 100.21° γ: 90°
R-values:
R R work R free
0.232 0.231 0.266
Expression system: Escherichia coli

Quick links

Citations

122 review citations

The control of DNA repair by the cell cycle.
Hustedt et al. (2016)
121 more

2 mentions without citation

Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks.
Tisi et al. (2020)
1 more