› 4o0n

X-ray diffraction
2.4Å resolution

2.4 Angstrom Resolution Crystal Structure of Putative Nucleoside Diphosphate Kinase from Toxoplasma gondii.

Released:
DOI: 10.2210/pdb4o0n/pdb
PDB entry 4o0n coloured by chain, front view.
PDB entry 4o0n coloured by chain, side view.
PDB entry 4o0n coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii.
Lykins JD, Filippova EV, Halavaty AS, Minasov G, Zhou Y, Dubrovska I, Flores KJ, Shuvalova LA, Ruan J, El Bissati K, Dovgin S, Roberts CW, Woods S, Moulton JD, Moulton H, McPhillie MJ, Muench SP, Fishwick CWG, Sabini E, Shanmugam D, Roos DS, McLeod R, Anderson WF, Ngô HM
OpenAccess logo Front Cell Infect Microbiol 8 352 (2018)
PMID: 30345257

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 171 amino acids
Theoretical weight: 19.56 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9Q287 (Residues: 1-155; Coverage: 100%)
Gene names: BN1205_050530, TGVEG_295350
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 73.385Å b: 121.591Å c: 212.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Treatment of toxoplasmosis: Current options and future perspectives.
Konstantinovic et al. (2019)
3 more

1 mention without citation

CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii.
Lykins et al. (2018)