› 4o9d

X-ray diffraction
2Å resolution

Structure of Dos1 propeller

Released:
DOI: 10.2210/pdb4o9d/pdb
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PDB entry 4o9d coloured by chain, front view.
PDB entry 4o9d coloured by chain, side view.
PDB entry 4o9d coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing.
Kuscu C, Zaratiegui M, Kim HS, Wah DA, Martienssen RA, Schalch T, Joshua-Tor L
Proc Natl Acad Sci U S A 111 1795-800 (2014)
PMID: 24449894

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rik1-associated factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 47.41 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O74910 (Residues: 213-638; Coverage: 67%)
Gene names: SPCC613.12c, clr8, cmc1, dos1, raf1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand EDO 9 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 121.15Å b: 102.79Å c: 95.1Å
α: 90° β: 122.36° γ: 90°
R-values:
R R work R free
0.214 0.213 0.25
Expression system: Trichoplusia ni

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Citations

5 review citations

Histone Methylation by SET Domain Proteins in Fungi.
Freitag M. (2017)
4 more

1 mention without citation

CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing.
Kuscu et al. (2014)

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