› 4ope

X-ray diffraction
2.58Å resolution

Streptomcyes albus JA3453 oxazolomycin ketosynthase domain OzmH KS7

Released:
DOI: 10.2210/pdb4ope/pdb
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Source organism: Streptomyces albus
Primary publication:
Structural and evolutionary relationships of "AT-less" type I polyketide synthase ketosynthases.
Lohman JR, Ma M, Osipiuk J, Nocek B, Kim Y, Chang C, Cuff M, Mack J, Bigelow L, Li H, Endres M, Babnigg G, Joachimiak A, Phillips GN, Shen B
Proc Natl Acad Sci U S A 112 12693-8 (2015)
PMID: 26420866

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NRPS/PKS Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 587 amino acids
Theoretical weight: 62.54 KDa
Source organism: Streptomyces albus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2WW42 (Residues: 4221-4804; Coverage: 8%)
Gene name: ozmH
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand NO3 2 x NO3
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 135.312Å b: 139.834Å c: 173.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Evolution and Diversity of Assembly-Line Polyketide Synthases.
Nivina et al. (2019)
10 more

2 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
1 more

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