› 4otm

X-ray diffraction
1.95Å resolution

Crystal structure of the C-terminal domain from yeast GCN2

Released:
DOI: 10.2210/pdb4otm/pdb
PDB entry 4otm coloured by chain, front view.
PDB entry 4otm coloured by chain, side view.
PDB entry 4otm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structures of GCN2 protein kinase C-terminal domains suggest regulatory differences in yeast and mammals.
He H, Singh I, Wek SA, Dey S, Baird TD, Wek RC, Georgiadis MM
J Biol Chem 289 15023-34 (2014)
PMID: 24719324

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
eIF-2-alpha kinase GCN2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 15.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15442 (Residues: 1519-1659; Coverage: 9%)
Gene names: AAS1, GCN2, YDR283C
Sequence domains: Anticodon binding domain of tRNAs

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21212
Unit cell:
a: 47.126Å b: 118.896Å c: 46.608Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.275
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Towards a model of GCN2 activation.
Masson GR. (2019)
1 more

1 mention without citation

Crystal structures of GCN2 protein kinase C-terminal domains suggest regulatory differences in yeast and mammals.
He et al. (2014)