› 4owj

X-ray diffraction
2Å resolution

Crystal Structure of the Vibrio vulnificus Hemolysin/Cytolysin Beta-Trefoil Lectin

Released:
DOI: 10.2210/pdb4owj/pdb
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PDB entry 4owj coloured by chain, top view.
Source organism: Vibrio vulnificus CMCP6
Primary publication:
Glycan specificity of the Vibrio vulnificus hemolysin lectin outlines evolutionary history of membrane targeting by a toxin family.
Kaus K, Lary JW, Cole JL, Olson R
J Mol Biol 426 2800-12 (2014)
PMID: 24862282

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo heptamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytolysin Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 138 amino acids
Theoretical weight: 15.42 KDa
Source organism: Vibrio vulnificus CMCP6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19247 (Residues: 338-471; Coverage: 30%)
Gene names: VV2_0404, vvhA
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 75.833Å b: 118.214Å c: 149.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.2
Expression system: Escherichia coli BL21

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Citations

7 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
6 more

2 mentions without citation

Structural Basis of the Pore-Forming Toxin/Membrane Interaction.
Li et al. (2021)
1 more

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