› 4oxr

X-ray diffraction
2Å resolution

Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Manganese

Released:
DOI: 10.2210/pdb4oxr/pdb
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Source organism: Staphylococcus pseudintermedius
Primary publication:
Apo, Zn2+-bound and Mn2+-bound structures reveal ligand-binding properties of SitA from the pathogen Staphylococcus pseudintermedius.
Abate F, Malito E, Cozzi R, Lo Surdo P, Maione D, Bottomley MJ
OpenAccess logo Biosci Rep 34 e00154 (2014)
PMID: 25311310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese ABC transporter, periplasmic-binding protein SitA Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.19 KDa
Source organism: Staphylococcus pseudintermedius
Expression system: Escherichia coli BL21(DE3)
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Unit cell:
a: 80.58Å b: 57.49Å c: 112.349Å
α: 90° β: 101.95° γ: 90°
R-values:
R R work R free
0.186 0.183 0.233
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Lipoproteins of Gram-Positive Bacteria: Key Players in the Immune Response and Virulence.
Nguyen et al. (2016)
6 more

3 mentions without citation

Apo, Zn2+-bound and Mn2+-bound structures reveal ligand-binding properties of SitA from the pathogen Staphylococcus pseudintermedius.
Abate et al. (2014)
2 more

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