› 4ozq

X-ray diffraction
2.71Å resolution

Crystal structure of the mouse Kif14 motor domain

Released:
DOI: 10.2210/pdb4ozq/pdb
PDB entry 4ozq coloured by chain, front view.
PDB entry 4ozq coloured by chain, side view.
PDB entry 4ozq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
KIF14 binds tightly to microtubules and adopts a rigor-like conformation.
Arora K, Talje L, Asenjo AB, Andersen P, Atchia K, Joshi M, Sosa H, Allingham JS, Kwok BH
J Mol Biol 426 2997-3015 (2014)
PMID: 24949858

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 720 amino acids
Theoretical weight: 79.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: L0N7N1 (Residues: 390-738; Coverage: 21%)
Gene name: Kif14
Sequence domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P1
Unit cell:
a: 65.137Å b: 72.193Å c: 94.098Å
α: 74.43° β: 87.45° γ: 89.94°
R-values:
R R work R free
0.266 0.264 0.308
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Dissecting the Genetic and Etiological Causes of Primary Microcephaly.
Jean et al. (2020)
6 more

9 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
8 more