› 4p3h

X-ray diffraction
1.45Å resolution

Crystal structure of Kaposi's sarcoma-associated herpesvirus (KSHV) protease in complex with dimer disruptor

Released:
DOI: 10.2210/pdb4p3h/pdb
PDB entry 4p3h coloured by chain, front view.
PDB entry 4p3h coloured by chain, side view.
PDB entry 4p3h coloured by chain, top view.
Source organism: Human gammaherpesvirus 8
Primary publication:
Broad-spectrum allosteric inhibition of herpesvirus proteases.
Gable JE, Lee GM, Jaishankar P, Hearn BR, Waddling CA, Renslo AR, Craik CS
OpenAccess logo Biochemistry 53 4648-60 (2014)
PMID: 24977643

Function and Biology Details

Reaction catalysed: 
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-558022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Assemblin Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.19 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2HRB6 (Residues: 4-196; Coverage: 36%)
Gene name: ORF17
Sequence domains: Assemblin (Peptidase family S21)
Structure domains: Herpesvirus/Caudovirus protease domain

Ligands and Environments

3 bound ligands:
Ligand 25G 3 x 25G
Ligand GOL 1 x GOL
Ligand DMS 2 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I222
Unit cell:
a: 69.12Å b: 95.892Å c: 119.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.195
Expression system: Escherichia coli

Quick links

Citations

5 review citations

KSHV targeted therapy: an update on inhibitors of viral lytic replication.
Coen et al. (2014)
4 more

6 mentions without citation

Broad-spectrum allosteric inhibition of herpesvirus proteases.
Gable et al. (2014)
5 more