› 4p53

X-ray diffraction
2.1Å resolution

ValA (2-epi-5-epi-valiolone synthase) from Streptomyces hygroscopicus subsp. jinggangensis 5008 with NAD+ and Zn2+ bound

Released:
DOI: 10.2210/pdb4p53/pdb
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PDB entry 4p53 coloured by chain, front view.
PDB entry 4p53 coloured by chain, side view.
PDB entry 4p53 coloured by chain, top view.
Source organism: Streptomyces hygroscopicus subsp. jinggangensis 5008
Primary publication:
Structure of a sedoheptulose 7-phosphate cyclase: ValA from Streptomyces hygroscopicus.
Kean KM, Codding SJ, Asamizu S, Mahmud T, Karplus PA
OpenAccess logo Biochemistry 53 4250-60 (2014)
PMID: 24832673

Function and Biology Details

Reaction catalysed: 
D-sedoheptulose 7-phosphate = 2-epi-5-epi-valiolone + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-epi-5-epi-valiolone synthase Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 45.9 KDa
Source organism: Streptomyces hygroscopicus subsp. jinggangensis 5008
Expression system: Escherichia coli
UniProt:
  • Canonical: H2K887 (Residues: 1-414; Coverage: 100%)
Gene names: SHJG_0276, valA
Sequence domains: 3-dehydroquinate synthase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 1 x NAI
2 bound ligands:
Ligand ZN 1 x ZN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 77.186Å b: 77.186Å c: 99.074Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.179 0.262
Expression system: Escherichia coli

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Citations

2 review citations

Assessing and maximizing data quality in macromolecular crystallography.
Karplus et al. (2015)
1 more

6 mentions without citation

Predicting Structural Details of the Sodium Channel Pore Basing on Animal Toxin Studies.
Tikhonov et al. (2018)
5 more

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