› 4plw

X-ray diffraction
1.85Å resolution

Crystal structure of ancestral apicomplexan malate dehydrogenase with lactate.

Released:
DOI: 10.2210/pdb4plw/pdb
PDB entry 4plw coloured by chain, front view.
PDB entry 4plw coloured by chain, side view.
PDB entry 4plw coloured by chain, top view.
Source organism: Apicomplexa
Primary publication:
An atomic-resolution view of neofunctionalization in the evolution of apicomplexan lactate dehydrogenases.
Boucher JI, Jacobowitz JR, Beckett BC, Classen S, Theobald DL
OpenAccess logo Elife 3 (2014)
PMID: 24966208

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 326 amino acids
Theoretical weight: 35.81 KDa
Source organism: Apicomplexa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075B5H2 (Residues: 1-326; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 4 x NAI
1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P212121
Unit cell:
a: 90.367Å b: 103.738Å c: 143.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

A mechanistic view of enzyme evolution.
Yang et al. (2020)
2 more

1 mention without citation

An atomic-resolution view of neofunctionalization in the evolution of apicomplexan lactate dehydrogenases.
Boucher et al. (2014)