› 4pmw

X-ray diffraction
2.95Å resolution

Structure of mouse Dis3L2 in complex with oligoU RNA substrate

Released:
DOI: 10.2210/pdb4pmw/pdb
PDB entry 4pmw coloured by chain, front view.
PDB entry 4pmw coloured by chain, side view.
PDB entry 4pmw coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of Dis3l2 substrate recognition in the Lin28-let-7 pathway.
Faehnle CR, Walleshauser J, Joshua-Tor L
OpenAccess logo Nature 514 252-256 (2014)
PMID: 25119025

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183977 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DIS3-like exonuclease 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 770 amino acids
Theoretical weight: 87.12 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8CI75 (Residues: 37-856; Coverage: 89%)
Gene name: Dis3l2
Sequence domains:
Structure domains:
U-U-U-U-U-U-U-U-U-U-U-U-U-U Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.24 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 63.927Å b: 96.075Å c: 157.311Å
α: 90° β: 98.75° γ: 90°
R-values:
R R work R free
0.205 0.202 0.251
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

29 review citations

MicroRNA biogenesis pathways in cancer.
Lin et al. (2015)
28 more

8 mentions without citation

Mechanism of Dis3l2 substrate recognition in the Lin28-let-7 pathway.
Faehnle et al. (2014)
7 more