4pog

X-ray diffraction
3.2Å resolution

MCM-ssDNA co-crystal structure

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-508857 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA helicase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 257 amino acids
Theoretical weight: 29.4 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3I4 (Residues: 2-256; Coverage: 24%)
Gene name: PF0482
Sequence domains:
Structure domains:
30-mer oligo(dT) Chains: V, X, Y, Z
Molecule details ›
Chains: V, X, Y, Z
Length: 30 nucleotides
Theoretical weight: 9.08 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 94.277Å b: 113.398Å c: 196.855Å
α: 90° β: 101.35° γ: 90°
R-values:
R R work R free
0.259 0.257 0.294
Expression systems:
  • Escherichia coli
  • Not provided