› 4pu2

X-ray diffraction
2.1Å resolution

Crystal structure of Aminopeptidase N in complex with the phosphonic acid analogue of leucine L-(R)-LeuP

Released:
DOI: 10.2210/pdb4pu2/pdb
PDB entry 4pu2 coloured by chain, front view.
PDB entry 4pu2 coloured by chain, side view.
PDB entry 4pu2 coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Entry authors: Nocek B, Vassiliou S, Berlicki L, Mulligan R, Mucha A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminopeptidase N Chain: A
Molecule details ›
Chain: A
Length: 867 amino acids
Theoretical weight: 98.69 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JYV4 (Residues: 1-867; Coverage: 100%)
Gene names: NMB1416, pepN
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PLU 1 x PLU
Ligand GOL 2 x GOL
Ligand ZN 1 x ZN
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 19 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 224.328Å b: 224.328Å c: 57.909Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli

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