› 4qht

X-ray diffraction
2.56Å resolution

Crystal structure of AAA+/ sigma 54 activator domain of the flagellar regulatory protein FlrC from Vibrio cholerae in ATP analog bound state

Released:
DOI: 10.2210/pdb4qht/pdb
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Source organism: Vibrio cholerae O395
Primary publication:
Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC.
Dey S, Biswas M, Sen U, Dasgupta J
J Biol Chem 290 8734-47 (2015)
PMID: 25688103

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar regulatory protein C Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 267 amino acids
Theoretical weight: 29.83 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3AHP1 (Residues: 132-381; Coverage: 52%)
Gene names: VC0395_A1719, flrC
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 7 x ANP
Ligand MG 7 x MG
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 80.959Å b: 152.56Å c: 193.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.247
Expression system: Escherichia coli

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Citations

3 review citations

Vibrio Flagellar Synthesis.
Echazarreta et al. (2019)
2 more

2 mentions without citation

Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC.
Dey et al. (2015)
1 more

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