› 4qpj

X-ray diffraction
2.74Å resolution

2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain

Released:
DOI: 10.2210/pdb4qpj/pdb
Model geometry
Fit model/data
PDB entry 4qpj coloured by chain, front view.
PDB entry 4qpj coloured by chain, side view.
PDB entry 4qpj coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen.
Willett JW, Herrou J, Briegel A, Rotskoff G, Crosson S
Proc Natl Acad Sci U S A 112 E3709-18 (2015)
PMID: 26124143

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174188 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein phosphotransferase ChpT Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 25.87 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQA5 (Residues: 1-209; Coverage: 100%)
Gene names: BAB1_1613, chpT
Sequence domains: Histidine phosphotransferase C-terminal domain
Structure domains:
Cell cycle response regulator CtrA Chains: C, D
Molecule details ›
Chains: C, D
Length: 152 amino acids
Theoretical weight: 16.87 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQA4 (Residues: 1-118; Coverage: 51%)
Gene names: BAB1_1614, ctrA
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

5 bound ligands:
Ligand GOL 5 x GOL
Ligand NA 1 x NA
Ligand CA 3 x CA
Ligand CL 1 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 124.951Å b: 124.951Å c: 136.332Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.175 0.222
Expression system: Escherichia coli

Quick links

Citations

9 review citations

VAP, a Versatile Access Point for the Endoplasmic Reticulum: Review and analysis of FFAT-like motifs in the VAPome.
Murphy et al. (2016)
8 more

8 mentions without citation

Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen.
Willett et al. (2015)
7 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO