› 4rnf

X-ray diffraction
2.85Å resolution

PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form

Released:
DOI: 10.2210/pdb4rnf/pdb
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PDB entry 4rnf coloured by chain, front view.
PDB entry 4rnf coloured by chain, side view.
PDB entry 4rnf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator.
Phippen CW, Mikolajek H, Schlaefli HG, Keevil CW, Webb JS, Tews I
FEBS Lett 588 4631-6 (2014)
PMID: 25447517

Function and Biology Details

Reactions catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Motility regulator Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 50.45 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVI8 (Residues: 978-1409; Coverage: 31%)
Gene names: PA4601, morA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 40.744Å b: 47.352Å c: 60.01Å
α: 82.15° β: 71.63° γ: 70.66°
R-values:
R R work R free
0.241 0.239 0.278
Expression system: Escherichia coli

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Citations

2 review citations

C-di-GMP Synthesis: Structural Aspects of Evolution, Catalysis and Regulation.
Schirmer T. (2016)
1 more

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