› 4rx6

X-ray diffraction
2.6Å resolution

Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom

Released:
DOI: 10.2210/pdb4rx6/pdb
PDB entry 4rx6 coloured by chain, front view.
PDB entry 4rx6 coloured by chain, side view.
PDB entry 4rx6 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis.
Schumacher MA, Chinnam NB, Cuthbert B, Tonthat NK, Whitfill T
OpenAccess logo Genes Dev 29 451-64 (2015)
PMID: 25691471

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory PII-like protein Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 121 amino acids
Theoretical weight: 13.4 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07428 (Residues: 1-116; Coverage: 100%)
Gene names: BSU36520, nrgB
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand ATP 3 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 103.2Å b: 103.2Å c: 77.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.224 0.271
Expression system: Escherichia coli

Quick links

Citations

5 review citations

A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Commichau et al. (2015)
4 more

1 mention without citation

Prediction of Protein-ATP Binding Residues Based on Ensemble of Deep Convolutional Neural Networks and LightGBM Algorithm.
Song et al. (2021)