› 4rz4

X-ray diffraction
1.75Å resolution

Fructose-6-phosphate aldolase Q59E Y131F from E.coli

Released:
DOI: 10.2210/pdb4rz4/pdb
PDB entry 4rz4 coloured by chain, front view.
PDB entry 4rz4 coloured by chain, side view.
PDB entry 4rz4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Acid Base Catalyst Discriminates between a Fructose 6-Phosphate Aldolase and a Transaldolase
Stellmacher L, Sandalova T, Leptihn S, Schneider G, Sprenger GA, Samland AK
ChemCatChem (2015)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-6-phosphate aldolase 1 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 226 amino acids
Theoretical weight: 23.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78055 (Residues: 2-220; Coverage: 100%)
Gene names: JW5109, b0825, fsa, fsaA, mipB, ybiZ
Sequence domains: Transaldolase/Fructose-6-phosphate aldolase
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand PGE 20 x PGE
Ligand PO4 10 x PO4
Ligand CL 10 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 88.44Å b: 133Å c: 102.27Å
α: 90° β: 92.37° γ: 90°
R-values:
R R work R free
0.18 0.179 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links