› 4rzs

X-ray diffraction
2.71Å resolution

Lac repressor engineered to bind sucralose, unliganded tetramer

Released:
DOI: 10.2210/pdb4rzs/pdb
PDB entry 4rzs coloured by chain, front view.
PDB entry 4rzs coloured by chain, side view.
PDB entry 4rzs coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
Engineering an allosteric transcription factor to respond to new ligands.
Taylor ND, Garruss AS, Moretti R, Chan S, Arbing MA, Cascio D, Rogers JK, Isaacs FJ, Kosuri S, Baker D, Fields S, Church GM, Raman S
Nat Methods 13 177-83 (2016)
PMID: 26689263

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 40.88 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-360; Coverage: 100%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 91.09Å b: 111.6Å c: 189.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.239
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Allostery in Its Many Disguises: From Theory to Applications.
Wodak et al. (2019)
38 more

2 mentions without citation

Engineering an allosteric transcription factor to respond to new ligands.
Taylor et al. (2016)
1 more