› 4s2p

X-ray diffraction
1.7Å resolution

Crystal structure of unbound OXA-48

Released:
DOI: 10.2210/pdb4s2p/pdb
PDB entry 4s2p coloured by chain, front view.
PDB entry 4s2p coloured by chain, side view.
PDB entry 4s2p coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Molecular Mechanism of Avibactam-Mediated β-Lactamase Inhibition.
King DT, King AM, Lal SM, Wright GD, Strynadka NC
ACS Infect Dis 1 175-84 (2015)
PMID: 27622530

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.44 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6XEC0 (Residues: 1-265; Coverage: 100%)
Gene names: BANRA_05332, BV369_20085, GJJ01_28680, KPE71T_00045, SAMEA3729652_05148, bla OXA-48, blaOXA-48, bla_4
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

No bound ligands
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Unit cell:
a: 43.41Å b: 102.87Å c: 124.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

New β-Lactamase Inhibitors in the Clinic.
Papp-Wallace et al. (2016)
6 more

10 mentions without citation

Resistance to Novel β-Lactam-β-Lactamase Inhibitor Combinations: The "Price of Progress".
Papp-Wallace et al. (2020)
9 more