› 4ua2

X-ray diffraction
2.61Å resolution

Crystal structure of dual function transcriptional regulator MerR from Bacillus megaterium MB1

Released:
DOI: 10.2210/pdb4ua2/pdb
PDB entry 4ua2 coloured by chain, front view.
PDB entry 4ua2 coloured by chain, side view.
PDB entry 4ua2 coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structural basis of the mercury(II)-mediated conformational switching of the dual-function transcriptional regulator MerR.
Chang CC, Lin LY, Zou XW, Huang CC, Chan NL
OpenAccess logo Nucleic Acids Res 43 7612-23 (2015)
PMID: 26150423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mercuric resistance operon regulatory protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 132 amino acids
Theoretical weight: 16.33 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q799U3 (Residues: 1-132; Coverage: 100%)
Gene names: merR, merR1
Sequence domains: MerR HTH family regulatory protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21
Unit cell:
a: 80.542Å b: 94.806Å c: 87.06Å
α: 90° β: 101.83° γ: 90°
R-values:
R R work R free
0.227 0.225 0.257
Expression system: Escherichia coli

Quick links

Citations

8 review citations

A New Facet of Vitamin B12: Gene Regulation by Cobalamin-Based Photoreceptors.
Padmanabhan et al. (2017)
7 more

1 mention without citation

Structural basis of the mercury(II)-mediated conformational switching of the dual-function transcriptional regulator MerR.
Chang et al. (2015)