› 4uca

X-ray diffraction
3.22Å resolution

N-terminal globular domain of the RSV Nucleoprotein in complex with C- terminal peptide of the Phosphoprotein

Released:
DOI: 10.2210/pdb4uca/pdb
PDB entry 4uca coloured by chain, front view.
PDB entry 4uca coloured by chain, side view.
PDB entry 4uca coloured by chain, top view.
Source organism: Human respiratory syncytial virus A2
Primary publication:
A Druggable Pocket at the Nucleocapsid/Phosphoprotein Interaction Site of Human Respiratory Syncytial Virus.
Ouizougun-Oubari M, Pereira N, Tarus B, Galloux M, Lassoued S, Fix J, Tortorici MA, Hoos S, Baron B, England P, Desmaële D, Couvreur P, Bontems F, Rey FA, Eléouët JF, Sizun C, Slama-Schwok A, Duquerroy S
J Virol 89 11129-43 (2015)
PMID: 26246564

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-136969 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.27 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03418 (Residues: 31-252; Coverage: 57%)
Gene name: N
Sequence domains: Pneumovirus nucleocapsid protein
Phosphoprotein Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 409 Da
Source organism: Human respiratory syncytial virus A2
Expression system: Not provided
UniProt:
  • Canonical: P03421 (Residues: 239-241; Coverage: 1%)
Gene name: P

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 33.84Å b: 71.58Å c: 177.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.229
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

11 review citations

Respiratory Syncytial Virus: Infection, Detection, and New Options for Prevention and Treatment.
Griffiths et al. (2017)
10 more

2 mentions without citation

Interactions between the Nucleoprotein and the Phosphoprotein of Pneumoviruses: Structural Insight for Rational Design of Antivirals.
Decool et al. (2021)
1 more