› 4uer

Electron Microscopy
6.47Å resolution

40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lachancea kluyveri

Released:
DOI: 10.2210/pdb4uer/pdb
PDB entry 4uer coloured by chain, front view.
PDB entry 4uer coloured by chain, side view.
PDB entry 4uer coloured by chain, top view.
Source organism: Lachancea kluyveri
Primary publication:
Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex.
Aylett CH, Boehringer D, Erzberger JP, Schaefer T, Ban N
Nat Struct Mol Biol 22 269-71 (2015)
PMID: 25664723
Related structures: EMD-2845

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 39-mer (preferred)
PDBe Complex ID:
PDB-CPX-102254 (preferred)
Entry contents:
38 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (39 distinct):
Eukaryotic translation initiation factor 4C Chain: 0
Molecule details ›
Chain: 0
Length: 100 amino acids
Theoretical weight: 11.51 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X202 (Residues: 1-100; Coverage: 100%)
Sequence domains: Translation initiation factor 1A / IF-1
ES28 Chain: 1
Molecule details ›
Chain: 1
Length: 63 amino acids
Theoretical weight: 7.12 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X212 (Residues: 1-63; Coverage: 100%)
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S8 Chain: 2
Molecule details ›
Chain: 2
Length: 188 amino acids
Theoretical weight: 21.15 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X221 (Residues: 1-188; Coverage: 100%)
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S7 Chain: 3
Molecule details ›
Chain: 3
Length: 184 amino acids
Theoretical weight: 21 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X232 (Residues: 1-184; Coverage: 100%)
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS1 Chain: 4
Molecule details ›
Chain: 4
Length: 214 amino acids
Theoretical weight: 24.31 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X233 (Residues: 1-214; Coverage: 100%)
Gene name: RPS1
Sequence domains: Ribosomal S3Ae family
40S ribosomal protein S26 Chain: 5
Molecule details ›
Chain: 5
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X234 (Residues: 1-97; Coverage: 100%)
Sequence domains: Ribosomal protein S26e
40S ribosomal protein S27 Chain: 6
Molecule details ›
Chain: 6
Length: 81 amino acids
Theoretical weight: 8.76 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X235 (Residues: 1-81; Coverage: 100%)
Sequence domains: Ribosomal protein S27
Plectin/eS10 N-terminal domain-containing protein Chain: 7
Molecule details ›
Chain: 7
Length: 96 amino acids
Theoretical weight: 11.57 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X236 (Residues: 1-96; Coverage: 100%)
Sequence domains: Plectin/S10 domain
40S ribosomal protein S25 Chain: 8
Molecule details ›
Chain: 8
Length: 70 amino acids
Theoretical weight: 8 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X237 (Residues: 1-70; Coverage: 100%)
Sequence domains: S25 ribosomal protein
Small ribosomal subunit protein eS31 domain-containing protein Chain: 9
Molecule details ›
Chain: 9
Length: 71 amino acids
Theoretical weight: 7.41 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X203 (Residues: 21-71; Coverage: 72%)
Sequence domains: Ribosomal protein S27a
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 206 amino acids
Theoretical weight: 22.81 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X204 (Residues: 1-206; Coverage: 100%)
Gene name: RPS0
Sequence domains: Ribosomal protein S2
40S ribosomal protein S3 Chain: C
Molecule details ›
Chain: C
Length: 223 amino acids
Theoretical weight: 24.7 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X205 (Residues: 1-223; Coverage: 100%)
Sequence domains:
US4 Chain: D
Molecule details ›
Chain: D
Length: 185 amino acids
Theoretical weight: 21.21 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X206 (Residues: 1-185; Coverage: 100%)
Sequence domains:
Small ribosomal subunit protein uS5 Chain: E
Molecule details ›
Chain: E
Length: 217 amino acids
Theoretical weight: 23.21 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X207 (Residues: 1-217; Coverage: 100%)
Sequence domains:
SUI1 domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 83 amino acids
Theoretical weight: 9.59 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X208 (Residues: 1-83; Coverage: 100%)
Sequence domains: Translation initiation factor SUI1
Small ribosomal subunit protein uS7 domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 206 amino acids
Theoretical weight: 22.91 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X209 (Residues: 1-206; Coverage: 100%)
Sequence domains: Ribosomal protein S7p/S5e
US8 Chain: H
Molecule details ›
Chain: H
Length: 129 amino acids
Theoretical weight: 14.52 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X210 (Residues: 1-129; Coverage: 100%)
Sequence domains: Ribosomal protein S8
US9 Chain: I
Molecule details ›
Chain: I
Length: 141 amino acids
Theoretical weight: 15.66 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X211 (Residues: 1-141; Coverage: 100%)
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 domain-containing protein Chain: J
Molecule details ›
Chain: J
Length: 107 amino acids
Theoretical weight: 12.19 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X213 (Residues: 1-107; Coverage: 100%)
Sequence domains: Ribosomal protein S10p/S20e
US11 Chain: K
Molecule details ›
Chain: K
Length: 127 amino acids
Theoretical weight: 13.45 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X214 (Residues: 1-127; Coverage: 100%)
Sequence domains: Ribosomal protein S11
RPS23 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.94 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: Q875Q2 (Residues: 2-145; Coverage: 99%)
Sequence domains: Ribosomal protein S12/S23
US13 Chain: M
Molecule details ›
Chain: M
Length: 145 amino acids
Theoretical weight: 16.94 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X215 (Residues: 1-145; Coverage: 100%)
Sequence domains: Ribosomal protein S13/S18
US14 Chain: N
Molecule details ›
Chain: N
Length: 53 amino acids
Theoretical weight: 6.34 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X216 (Residues: 1-53; Coverage: 100%)
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: O
Molecule details ›
Chain: O
Length: 150 amino acids
Theoretical weight: 16.93 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X217 (Residues: 1-150; Coverage: 100%)
Sequence domains:
40S ribosomal protein S24 Chain: P
Molecule details ›
Chain: P
Length: 134 amino acids
Theoretical weight: 15.23 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: Q875Q1 (Residues: 2-135; Coverage: 99%)
Sequence domains: Ribosomal protein S24e
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: Q
Molecule details ›
Chain: Q
Length: 155 amino acids
Theoretical weight: 17.65 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X218 (Residues: 1-155; Coverage: 100%)
Sequence domains:
Rack1 Chain: R
Molecule details ›
Chain: R
Length: 318 amino acids
Theoretical weight: 34.71 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X219 (Residues: 1-318; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
US19 Chain: S
Molecule details ›
Chain: S
Length: 124 amino acids
Theoretical weight: 14.16 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X220 (Residues: 1-124; Coverage: 100%)
Sequence domains: Ribosomal protein S19
ES19 Chain: T
Molecule details ›
Chain: T
Length: 143 amino acids
Theoretical weight: 15.81 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X222 (Residues: 1-143; Coverage: 100%)
Sequence domains: Ribosomal protein S19e
40S ribosomal protein S12 Chain: U
Molecule details ›
Chain: U
Length: 124 amino acids
Theoretical weight: 13.33 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X223 (Residues: 1-124; Coverage: 100%)
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
ES17 Chain: V
Molecule details ›
Chain: V
Length: 120 amino acids
Theoretical weight: 13.77 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X224 (Residues: 1-120; Coverage: 100%)
Sequence domains: Ribosomal S17
40S ribosomal protein S4 Chain: W
Molecule details ›
Chain: W
Length: 260 amino acids
Theoretical weight: 29.34 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X225 (Residues: 1-260; Coverage: 100%)
Sequence domains:
40S ribosomal protein S30 Chain: X
Molecule details ›
Chain: X
Length: 60 amino acids
Theoretical weight: 6.78 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X226 (Residues: 1-60; Coverage: 100%)
Sequence domains: Ribosomal protein S30
40S ribosomal protein S6 Chain: Y
Molecule details ›
Chain: Y
Length: 226 amino acids
Theoretical weight: 25.9 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X227 (Residues: 1-226; Coverage: 100%)
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S21 Chain: Z
Molecule details ›
Chain: Z
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X228 (Residues: 1-87; Coverage: 100%)
Sequence domains: Ribosomal protein S21e
Eukaryotic translation initiation factor 3 subunit A Chain: a
Molecule details ›
Chain: a
Length: 964 amino acids
Theoretical weight: 110.52 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X229 (Residues: 1-964; Coverage: 100%)
Gene name: TIF32
Sequence domains: eIF3a, PCI domain, TPR-like region
Eukaryotic translation initiation factor 3 subunit B Chain: b
Molecule details ›
Chain: b
Length: 763 amino acids
Theoretical weight: 88.24 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X230 (Residues: 1-763; Coverage: 100%)
Gene name: PRT1
Sequence domains:
Eukaryotic translation initiation factor 3 subunit C Chain: c
Molecule details ›
Chain: c
Length: 812 amino acids
Theoretical weight: 93.31 KDa
Source organism: Lachancea kluyveri
UniProt:
  • Canonical: A0A0J9X231 (Residues: 1-812; Coverage: 100%)
Gene name: NIP1
Sequence domains:
18S Ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1781 nucleotides
Theoretical weight: 571.94 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.47Å
Relevant EMDB volumes: EMD-2845

Quick links

Citations

18 review citations

Protein Synthesis Initiation in Eukaryotic Cells.
Merrick et al. (2018)
17 more

1 mention without citation

Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex.
Llácer et al. (2015)