› 4uey

X-ray diffraction
1.9Å resolution

Structure of the periplasmic domain PhoQ double mutant (W104C-A128C)

Released:
DOI: 10.2210/pdb4uey/pdb
PDB entry 4uey coloured by chain, front view.
PDB entry 4uey coloured by chain, side view.
PDB entry 4uey coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence.
Hicks KG, Delbecq SP, Sancho-Vaello E, Blanc MP, Dove KK, Prost LR, Daley ME, Zeth K, Klevit RE, Miller SI
OpenAccess logo Elife 4 e06792 (2015)
PMID: 26002083

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Virulence sensor histidine kinase PhoQ Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 17.84 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DM80 (Residues: 45-190; Coverage: 30%)
Gene names: STM1230, phoQ, phoZ
Sequence domains: PhoQ Sensor
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 128.035Å b: 45.371Å c: 81.374Å
α: 90° β: 102.53° γ: 90°
R-values:
R R work R free
0.232 0.231 0.266
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Bacterial Stress Responses during Host Infection.
Fang et al. (2016)
4 more

2 mentions without citation

Regulating polymyxin resistance in Gram-negative bacteria: roles of two-component systems PhoPQ and PmrAB.
Huang et al. (2020)
1 more