› 4uqt

Solution NMR

RRM-peptide structure in RES complex

Released:
DOI: 10.2210/pdb4uqt/pdb
PDB entry 4uqt coloured by chain, ensemble of 20 models, front view.
PDB entry 4uqt coloured by chain, ensemble of 20 models, side view.
PDB entry 4uqt coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A novel protein-protein interaction in the RES (REtention and Splicing) complex.
Tripsianes K, Friberg A, Barrandon C, Brooks M, van Tilbeurgh H, Seraphin B, Sattler M
OpenAccess logo J Biol Chem 289 28640-50 (2014)
PMID: 25160624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154144 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
U2 snRNP component IST3 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40565 (Residues: 25-113; Coverage: 60%)
Gene names: IST3, SNU17, YIB5W, YIR005W
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Pre-mRNA-splicing factor CWC26 Chain: B
Molecule details ›
Chain: B
Length: 39 amino acids
Theoretical weight: 4.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46947 (Residues: 222-256; Coverage: 13%)
Gene names: BUD13, CWC26, G1642, SLC7, YGL174W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.
Loerch et al. (2016)
13 other articles

3 mentions without citation

A novel protein-protein interaction in the RES (REtention and Splicing) complex.
Tripsianes et al. (2014)
2 more