› 4uwm

X-ray diffraction
1.9Å resolution

Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.

Released:
DOI: 10.2210/pdb4uwm/pdb
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Source organism: Pseudomonas putida
Primary publication:
The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase.
Isupov MN, Schröder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, Lau PC, Hasegawa Y, Iwaki H, Kadow M, Balke K, Bornscheuer UT, Bourenkov G, Littlechild JA
OpenAccess logo Acta Crystallogr D Biol Crystallogr 71 2344-53 (2015)
PMID: 26527149

Function and Biology Details

Reaction catalysed: 
(-)-bornane-2,5-dione + O(2) + FMNH(2) = (-)-5-oxo-1,2-campholide + FMN + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,6-diketocamphane 1,6-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 42.36 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D7UER1 (Residues: 1-378; Coverage: 100%)
Gene name: camE36
Sequence domains: Luciferase-like monooxygenase
Structure domains: Luciferase-like domain

Ligands and Environments

4 bound ligands:
Ligand FMN 2 x FMN
Ligand PG4 3 x PG4
Ligand PEG 4 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 72.79Å b: 82.84Å c: 149.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Baeyer-Villiger oxidations: biotechnological approach.
Bučko et al. (2016)
1 more

6 mentions without citation

Characterised Flavin-Dependent Two-Component Monooxygenases from the CAM Plasmid of Pseudomonas putida ATCC 17453 (NCIMB 10007): ketolactonases by Another Name.
Willetts A. (2018)
5 more

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