› 4vhb

X-ray diffraction
1.8Å resolution

THIOCYANATE ADDUCT OF THE BACTERIAL HEMOGLOBIN FROM VITREOSCILLA SP.

Released:
DOI: 10.2210/pdb4vhb/pdb
PDB entry 4vhb coloured by chain, front view.
PDB entry 4vhb coloured by chain, side view.
PDB entry 4vhb coloured by chain, top view.
Source organism: Vitreoscilla stercoraria
Primary publication:
Anticooperative ligand binding properties of recombinant ferric Vitreoscilla homodimeric hemoglobin: a thermodynamic, kinetic and X-ray crystallographic study.
Bolognesi M, Boffi A, Coletta M, Mozzarelli A, Pesce A, Tarricone C, Ascenzi P
J Mol Biol 291 637-50 (1999)
PMID: 10448042

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial hemoglobin Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 15.79 KDa
Source organism: Vitreoscilla stercoraria
Expression system: Escherichia coli
UniProt:
  • Canonical: P04252 (Residues: 1-146; Coverage: 100%)
Gene name: vhb
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand SCN 2 x SCN
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 62.62Å b: 40.38Å c: 62.78Å
α: 90° β: 105.93° γ: 90°
R-values:
R R work R free
0.199 not available not available
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial hemoglobins and flavohemoglobins: versatile proteins and their impact on microbiology and biotechnology.
Frey et al. (2003)
4 more

3 mentions without citation

Evolutionary plasticity of protein families: coupling between sequence and structure variation.
Panchenko et al. (2005)
2 more