› 4wal

X-ray diffraction
2.2Å resolution

Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A

Released:
DOI: 10.2210/pdb4wal/pdb
PDB entry 4wal coloured by chain, front view.
PDB entry 4wal coloured by chain, side view.
PDB entry 4wal coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.
Jacewicz A, Chico L, Smith P, Schwer B, Shuman S
OpenAccess logo RNA 21 401-14 (2015)
PMID: 25587180

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexadecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-171312 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Branchpoint-bridging protein Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12186 (Residues: 144-271; Coverage: 27%)
Gene names: BBP, L2949, MSL5, SF1, YLR116W
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
RNA (5'-R(P*UP*AP*CP*UP*AP*AP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P422
Unit cell:
a: 81.952Å b: 81.952Å c: 51.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.215
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.
DeHaven et al. (2016)
1 more

1 mention without citation

Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.
Jacewicz et al. (2015)