› 4wby

X-ray diffraction
1.5Å resolution

tRNA-processing enzyme (apo form I)

Released:
DOI: 10.2210/pdb4wby/pdb
PDB entry 4wby coloured by chain, front view.
PDB entry 4wby coloured by chain, side view.
PDB entry 4wby coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Measurement of Acceptor-TΨC Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.
Yamashita S, Martinez A, Tomita K
Structure 23 830-842 (2015)
PMID: 25914059

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
A-adding tRNA nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 48.23 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66728 (Residues: 428-824; Coverage: 48%)
Gene name: aq_411
Sequence domains: Poly A polymerase head domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 54.51Å b: 67.74Å c: 127.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
3 more