› 4we0

X-ray diffraction
2.1Å resolution

JC Polyomavirus VP1 five-fold pore mutant P223M

Released:
DOI: 10.2210/pdb4we0/pdb
PDB entry 4we0 coloured by chain, front view.
PDB entry 4we0 coloured by chain, side view.
PDB entry 4we0 coloured by chain, top view.
Source organism: JC polyomavirus
Primary publication:
Modulation of a pore in the capsid of JC polyomavirus reduces infectivity and prevents exposure of the minor capsid proteins.
Nelson CD, Ströh LJ, Gee GV, O'Hara BA, Stehle T, Atwood WJ
J Virol 89 3910-21 (2015)
PMID: 25609820

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major capsid protein VP1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 272 amino acids
Theoretical weight: 30.11 KDa
Source organism: JC polyomavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03089 (Residues: 23-290; Coverage: 76%)
Sequence domains: Polyomavirus coat protein
Structure domains: Capsid protein VP1,Polyomavirus

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 149.72Å b: 95.63Å c: 128.51Å
α: 90° β: 110.4° γ: 90°
R-values:
R R work R free
0.182 0.18 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The biology of JC polyomavirus.
Assetta et al. (2017)
2 more

1 mention without citation

Modulation of a pore in the capsid of JC polyomavirus reduces infectivity and prevents exposure of the minor capsid proteins.
Nelson et al. (2015)