› 4wen

X-ray diffraction
1.89Å resolution

Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V2

Released:
DOI: 10.2210/pdb4wen/pdb
PDB entry 4wen coloured by chain, front view.
PDB entry 4wen coloured by chain, side view.
PDB entry 4wen coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.
Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H
OpenAccess logo Vet Res 46 14 (2015)
PMID: 25828907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146865 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
K88 fimbrial protein AC Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 28.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14190 (Residues: 40-283; Coverage: 93%)
Gene name: faeG
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.75 KDa
Source organism: Lama glama
Expression system: Escherichia coli
UniProt:
  • Canonical: R9VYW2 (Residues: 1-121; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P321
Unit cell:
a: 145.833Å b: 145.833Å c: 37.875Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.226
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Evolution and structural dynamics of bacterial glycan binding adhesins.
Moonens et al. (2017)
7 other articles

3 mentions without citation

Single Domain Antibody application in bacterial infection diagnosis and neutralization.
Qin et al. (2022)
2 more