› 4wkp

X-ray diffraction
1.58Å resolution

Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with 2-(2-hydroxyethoxy)ethylthiomethyl-DADMe-Immucillin-A

Released:
DOI: 10.2210/pdb4wkp/pdb
PDB entry 4wkp coloured by chain, front view.
PDB entry 4wkp coloured by chain, side view.
PDB entry 4wkp coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
New Antibiotic Candidates against Helicobacter pylori.
Wang S, Cameron SA, Clinch K, Evans GB, Wu Z, Schramm VL, Tyler PC
J Am Chem Soc 137 14275-80 (2015)
PMID: 26494017

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 245 amino acids
Theoretical weight: 26.89 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZMY2 (Residues: 2-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand 3QA 4 x 3QA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 72.706Å b: 74.078Å c: 176.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Helicobacter pylori treatment in the post-antibiotics era-searching for new drug targets.
Roszczenko-Jasińska et al. (2020)
3 more

1 mention without citation

Selective Inhibitors of Helicobacter pylori Methylthioadenosine Nucleosidase and Human Methylthioadenosine Phosphorylase.
Harijan et al. (2019)